Discover genes that reverse cardiac ageing

GraphRAG Network Visualizer Cardiac GRN

Topological Cardiac GRN: Core transcription factors (GATA4, MEF2C, TBX5, NKX2-5) co-regulate structural sarcomere targets (TNNT2, MYH6, ACTC1) for cardiac rejuvenation.

Multi-Omics Perturbation Predictor (scGPT) Zero-Shot Mode

GATA4 Dosage: 1.0

MEF2C Dosage: 1.0

TBX5 Dosage: 1.0

NKX2-5 Dosage: 1.0

OCT4 (Pioneer): 0.0

SOX2 (Pioneer): 0.0

KLF4 (Pioneer): 0.0

NMN Booster: 0.0

MYC (Oncogene): 0.0

SNAI1 (EMT): 0.0

Oral Route (NMN gut microbiome deamidation penalty)

Semaglutide (GLP-1)

Omega-3 Fatty Acids

Plasmapheresis (Stress)

Decitabine (AML)

Ketamine Infusion

Bezisterim

Pitavastatin

Multivitamin

TIME-seq CpG Delta

Transcriptome Stability

Sirtuin Activity

Endothelial Rejuv

Syncytial Safety

AFRAID Frailty Age

Clinical Trial Outcomes & Provenance (Harvard Genetic Studies)

DunedinPACE Rate

1.000

Causal DamAge

0.00 Yrs

Causal AdaptAge

0.00 Yrs

Source: Adiv A. Johnson & Harvard Genetic Studies, Frontiers in Genetics, 2026. Systematic review of 41 human trials.

TIME-seq Epigenetic CpG Methylome Heatmap (100 Loci) Zenith v31 Engine

Youthful (0.0 Methylation)

Aged (1.0 Methylation)

Epigenetic & Structural Expression Profiles

mRNA-LNP Formulation Delivery Optimizer Encapsulation

Ionizable Molar %: 50.0%

Cholesterol Molar %: 38.5%

Helper Lipid Molar %: 10.0%

PEG-Lipid Molar %: 1.5%

N/P Ratio (Nitrogen/Phosphate): 6.0

Active Ligand Conjugation (Bypasses liver barrier)

PEG Molecular Weight (Da): 2000 Da

Encapsulation %

Cardiac Tropism

Liver Seq.

Endosomal Escape

Half-Life (t1/2)

Cytotoxicity

Status: -- Target: Heart (Cardiomyocytes)

Adjust parameters or toggle active targeting to resolve the continuous endothelial barrier.

NEUROS-X Arrhythmia Safety Engine 512-Node Cardiac Syncytium (ESI Conduction Substrate)

Audits candidate cocktails for in silico arrhythmia risks (Long QT, conduction block) across a 512-node cardiac syncytium substrate.

VERIFIED SAFE — STABLE CONDUCTION (512-NODE CARDIAC SYNCYTIUM)

Zenith v31.0 · ~2.42M Cells (1.94M Generalist + 486k Specialist) · Research Use Only

LIVE

⚡ AI Protocol Discovery

Zenith-102M Generic Manifold Research Use Only

Semantic Target

Reprogramming Mode

Gene Lookup UniProt Swiss-Prot

AF3 Results Enter Real Scores

VALIDATED

Population 2000

Engine Capacity

Zenith Ultra-v31.1

Session #----

Backend:

Online (Clinical)

Main Page

PAPER MAIN PAGE

Micro

PROTOCOL DESIGN Simulation controls

1. Reprogramming Factors

TARGET TRANSCRIPTION

H1FOO-DD (2024 Boost)

MPTR (Nilus Lab Rejuvenation)

Active Factors:

OCT4, SOX2, KLF4, c-MYC

2. Perturbations

3. CRISPR & ALPHAGENOME

98% Non-Coding AI

AlphaGenome 98% Non-Coding AI

Decode 98% non-coding genome & generate CRISPR repair sgRNA.

4. Color Analysis

5. Knowledge Integration

Upload papers or protocols. The AI will parse and apply them to the simulation.

Learned Knowledge

SCIENTIFIC PAPER

3. RENDERING

MICRO-VIEW CONTROLS

CYCLE: FLUORO / PHASE / IMMUNO

RESEARCH LABORATORY

Active Objective

Achieve >150 iPSCs with 0 Tumors

Scenario Presets

Translation Delay (Flux)

GENETIC NOISE 0.015

MUTATION PROBABILITY 0.05%

TISSUE COHESION (ORGANOIDS) OFF

PROTOCOL POTENCY 100%

Actin (Green)

Cytoplasm (Red)

DAPI (Blue)

LIVE TELEMETRY LIVE

Entropy--

Genomic Stability--

Predictive DriftSTABLE

Identity StabilitySECURE

Mean Mobility0.00 m/s

Target BioAge 0.1011

Barrier Fatigue NEUTRALIZED

SELECTED CELL

Cell ID--

Type--

Health--

BioAge--

Primary Marker (scVI) --

Epigenetic Stability --%

Signaling Intercept

GNN ACTIVE

Select a cell to intercept paracrine flux...

Microscopy Analysis

COLONY RADIUS 380px

CELL DENSITY 1800

CHANNEL: 488nm (GREEN) 90%

CHANNEL: DAPI (BLUE) 70%

EXPOSURE (BRIGHTNESS) 50%

Phalloidin (Actin Filaments)

mCherry (Cytoplasm)

DAPI (Nuclear DNA)

ANALYTICS

POPULATION COUNT (CURRENT)

Somatic iPSC Neuro Cardio Endo

TIME COURSE (60S)

ZENITH v31.0: 6K-GENOME EXPLORER

OCT4

SOX2

KLF4

MYC

LIN28

NANOG

CDX2

GATA6

TBX5

MEF2C

HAND2

NKX2.5

MAP2

PAX6

NESTIN

TUBB3

OBJECTIVE: 40X OIL IMMERSION
EXPOSURE: 180ms
PROVENANCE: PREDICTED (Zenith-v31.0)
STATUS: SCANNING COLONY... 60 FPS

AI PROTOCOL DISCOVERY Predicted

Gene Lookup — UniProt Swiss-Prot

Structure Validation — Boltz-1 Scores (pLDDT / pTM)

Expert Pharmacology Console

Pharmacology Mode: Synergistic Cascade

Genomic Anchor Profile

pLDDT > 90

Clinical Analytics & HD GRN

Population Count (Current)

Time Course (60s)

High-Res Transcriptome (scVI) Measured Data

IDLE

1.94M Generalist

Select a cell...

486K Specialist

Select a cell...

Zenith v31.0: 6K-Gene Foundation Explorer

Latent Exploratory Archive

Troubleshoot: Open API Documentation

MODE: HIGH_FIDELITY_MICROSCOPY

60 FPS

RESEARCH USE ONLY - Phase 1 Validation

TEMPORAL STATE AUDIT

Comparing T=0 (Baseline) vs. T=Current (Intervention)

Longitudinal Longevity Analysis (RUO)

Patient Digital Twin (Predicted): Zenith-Sim v31.0 | Clinical Profile: #4922-A (Research Use Only)

Current Biological Age

Baseline Stabilized

Model ELBO

305.2

[MODEL: scVI reconstruction loss on held-out cells]

Reconstruction MSE

0.023 0.004

[MODEL: 5-fold cross-validation]

Longevity Trajectory (BioAge Over Time)

Cellular Composition

Clinical Observations

Simulating long-term steady state... capture snapshots to see trends.

Protocol Recommendation

Awaiting sufficient longitudinal data points...

Discover genes that reverse cardiac ageing

Clinical Safety Audit

Proteotoxic Stress (ProtParam)

Oncogenic Risk (ClinVar/ENCODE)

Nilus Lab Zenith v31.1

TEMPORAL STATE AUDIT

Longitudinal Longevity Analysis (RUO)

Nextflow Sequencing QC Monitor